Experimental protocols

Our current enrichment, medium and sampling protocols are on the Methods page and in the electroPioreactor repository. The one for Crymlyn Fen is written to be followed at home.

Earlier protocols written up on this site:

CO₂ sparging
Control testing between Pioreactors
Autoclaves and sterilisation, including what a pressure cooker can and can’t do

A pressure cooker on a gas hob being used to sterilise vials — 2023: a pressure cooker standing in for an autoclave. The autoclaves page explains why it’s a stopgap. Photo: AMYBO, CC BY-SA 4.0.

Before trying anything, please read our legal notes and disclaimer and risk assessments. Please do a proper risk assessment first: pressurised CO₂, electrolysis gases and live cultures all need one.

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