Everything we make is open source and developed in public on GitHub. Most of the recent work is on the electroPioreactor, an affordable electro-bioreactor for growing hydrogen-oxidising bacteria, and on the medium and methods that go with it.
electroPioreactor
A Pioreactor with electrodes in the culture and a CO₂ line. The Aseptic electroPioreactor (AEP) is being built and tested with Imperial College London and the University of Edinburgh under CARMA Hub funding; the cheaper mixed-culture electroPioreactor (MEP) is for enrichment and teaching.
CARMA Hub project
Affordable Aseptic Electro-Bioreactor for Reproducible HOB Research. Can two labs with the same open kit get the same results?
Medium, methods and literature
A chloride-free growth medium that doubles as the electrolyte, enrichment protocols for the lab and the kitchen table, and the reading behind them.
PioFlo
A flow-through Pioreactor, so the Pioreactor software can control a fermenter of any size. A first prototype has been run with kombucha.
OpenValve and other hardware
A 3D-printable solenoid pinch valve from Harvey Mudd College’s clinic team, the CO₂ gas train, holders and the small parts that make it all fit on a bench.
| What | Where |
|---|---|
| Designs, build guides, plugin, protocols, results | amy-bo/electroPioreactor on GitHub, mirrored on Radicle |
| Pioreactor plugin, on its own | amy-bo/pioreactor-electroPioreactor-plugin |
| Our changes to Pioreactor’s software and OS images | amy-bo/pioreactor and amy-bo/CustoPiZer, forks we send pull requests from |
| Discussion, questions and results as they happen | forum.amybo.org |
| Videos | YouTube |
| Reading list | Bibliography and the AMYBO Zotero group |
| This website | amy-bo/website |
Past projects holds our notes on equipment and first experiments from 2023 and 2024, and Background explains why we chose hydrogen-oxidising bacteria in the first place. Both are worth a read, but the pages above are where the current work is.
Text CC BY 4.0, images CC BY-SA 4.0 unless marked otherwise. Licences · Privacy
